Fig. 2: Examples displaying the importance of illumination and detection parameters on fluorescence image outcome. | npj Imaging

Fig. 2: Examples displaying the importance of illumination and detection parameters on fluorescence image outcome.

From: Reflect: reporting guidelines for preclinical, translational and clinical fluorescence molecular imaging studies

Fig. 2

A The effect of varied illumination profiles. Illumination through ground glass results in a heterogeneous illumination profile, excessively exciting the center of the image and lacking intensity at the edge of the image. Illumination through engineered diffusers results in a more homogeneous illumination profile, shown by similar fluorescence signal intensity emitted by the center and four corner wells. In (B), the fluorescence images obtained of the same phantom using a variety of settings show the importance of reporting on user-settings as adjustments, in this case gain and exposure time, yield changes in the output. All color bars represent 8-bit pixel intensity, in arbitrary units (a.u.); All images per panel have been normalized to the same global pixel intensity.

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