Fig. 8: In vivo vascular fluorescence imaging in mice following intravenous ICG or JAM317 administration.
From: Low molecular weight 4,4’-quinocyanines for in vivo NIR-II fluorescence imaging
In vivo fluorescence imaging of mouse vasculature following intravenous injection of ICG (A–C) or JAM317 (D–G). ICG imaged in the NIR-I window A, NIR-II with a 1000 nm long-pass filter B, and NIR-II with a 1250 nm long-pass filter C demonstrates progressively reduced signal and spatial resolution at longer wavelengths. JAM317 (40 nmol, eggPC liposomes) imaged with 1000 nm D and 1250 nm E long-pass filters shows enhanced vessel contrast and improved visualization of fine vascular structures at longer wavelengths. Contrast-enhanced images F, G were generated using ImageJ’s CLAHE plugin, with inverted images providing clear delineation of vascular networks. A supplemental video shows real-time biodistribution and vascular illumination following JAM317 injection.
