Fig. 2: pan-ASLM images of pan-expanded HeLa cells. | npj Imaging

Fig. 2: pan-ASLM images of pan-expanded HeLa cells.

From: pan-ASLM: Axially Swept Light Sheet Microscopy for Fast and High-Resolution Imaging of Expanded Samples

Fig. 2: pan-ASLM images of pan-expanded HeLa cells.

a A single FOV image of two pan-expanded HeLa cells stained with Atto 488 NHS ester. b Zoomed-in view of mitochondria showing clearly resolvable cristae. c Zoomed-in view of nucleolus showing dense fibrillar component (green arrow), fibrillar center (yellow arrow), and granular component (orange arrow). d Zoomed-in view of HeLa cell from the same dataset as (a). e Zoomed-in view showing nuclear pore complexes. f Zoomed-in view showing mitochondria (orange arrows). g Zoomed-in and contrast-adjusted view of the white box in (d) showing a centriole. h Zoomed-in view of the nuclear envelope showing nuclear pore complexes (blue arrows). i Zoomed-in view of putative annular lamellae in the cytoplasm. Scale bars are not corrected for the expansion factor. Scale bars: a, d–f 100 μm, b, g–i 10 μm, c 20 μm.

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