Fig. 4: 19F MRI signal and volumetric analysis of spleens and lymph nodes in tumor-bearing and non-tumor-bearing mice.
From: Linking tumor viability and immune infiltration with dual-nucleus MRI in preclinical models
A, B 19F MRI and ¹H/19F overlay showing increased spleen signal and splenomegaly in tumor-bearing mice. C Quantification of spleen volume by MRI in non-tumor-bearing and tumor-bearing mice D Ex vivo weight and E 19F content in spleens at day 26. F Correlation between spleen volume and 19F content. G Confocal imaging of spleen sections showing PFC co-localized with F4/80+ cells (yellow arrows), F4/80+ cells lacking PFC (green arrows), PFC+ in F4/80- cells (white arrows) (scale bar = 20 μm). H Representative 19F MRI signal in lymph nodes of non-tumor-bearing mice. I, J Comparison of ipsilateral vs. contralateral inguinal lymph nodes in tumor-bearing mice. K, L Comparison of lymph node volume and 19F content between tumor-bearing and non-tumor-bearing mice. M, N Ipsilateral inguinal lymph nodes in tumor-bearing mice show increased volume and 19F signal relative to contralateral nodes.
