Fig. 2: Chronic sleep restriction regulates the global proteome in cerebral cortex.

Volcano plots showing changes in global proteomes of SD/NS (A), SD/RS (B), and (C) RS/NS comparisons. Unpaired t-tests followed by false discovery rate (FDR) analysis were used to compare groups. Significantly upregulated and downregulated protein expression is color coded with red and blue, respectively (adjusted p-value < 0.2). Proteins in grey are not significantly changed after sleep deprivation. Bar plots showing significantly enriched top upregulated (D, E) and downregulated (F, G) GOBP and RGS, respectively. GOBP gene ontology biological process, RGS reactome gene sets. NS normal sleep, SD sleep deprived, RS recovery sleep, IGF insulin-like growth factor, IGFBPs insulin-like growth factor binding proteins.