Fig. 4: Methods for characterizing metabolite-protein interactions.
From: Regulation of gene expression through protein-metabolite interactions
Summary of metabolite-protein interaction profiling technique categorized in protein-centric, metabolite-centric and interactome-wide approaches. MIDAS mass spectrometry integrated with equilibrium dialysis for the discovery of allostery systematically, NMR nuclear magnetic resonance, AP-MS affinity purification-mass spectrometry, DARTS drug affinity responsive target stability, LiP-MS limited-proteolysis coupled to mass spectrometry, CETSA cellular thermal shift assay, TPP thermal proteome profiling, Tm melting temperature, PROMIS protein-metabolite interaction using size selection, LC-MS/MS liquid chromatography tandem mass spectrometry, AI artificial intelligence, SIMMER systematic identification of meaningful metabolic enzyme regulation.
