Fig. 2: Diagnostic properties of the nanobodies. | npj Biosensing

Fig. 2: Diagnostic properties of the nanobodies.

From: Nanobodies raised against the cytotoxic α-synuclein oligomer are oligomer-specific and promote its cellular uptake

Fig. 2: Diagnostic properties of the nanobodies.

The ability of nanobodies a NB1 and b NB2 to detect different species of α-Syn spiked into mouse brain lysates. c Control experiments. Different α-Syn species were used to coat wells, after which NB1 or NB2 was added. The signal was detected using an anti-His antibody (detects nanobody) or the anti α-Syn antibody 14-9E7 A1. d Brain tissues from 4 PD mice were used to coat wells in 1:1 or 1:5 dilution, after which the anti-α-Syn antibody MJF14 or nanobody was added. Background level (0.14 units) subtracted. Errors based on triplicates.

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