Table 2 Biophysical parameters for nanobodies NB1 and NB2 and their binding to αSO
Parameter | Technique | NB1 | NB2 |
|---|---|---|---|
[GdmCl]50% (M) | Fluorimeter | 1.96 ± 0.03 | 1.95 ± 0.015 |
tm (°C) | Far-UV CD | 65.4 ± 0.3 | 64.5 ± 0.3 |
tm (°C) | DSF | 66.8 ± 0.1 | 66.3 ± 0.15 |
KD for binding to αSO | FIDA | 31.3 ± 6.4 nM | 41.2 ± 4.9 nM |
Indicator size | FIDA | 2.23 ± 0.15 nm | 1.90 ± 0.07 nm |
Complex size | FIDA | 9.81 ± 0.44 nm | 9.83 ± 0.35 nm |
KD for binding to αSO | SPR | 3.2 ± 0.1 nM | 31 ± 1.2 nM |
