Fig. 2: Device-Free CONAN using disposable hand warmers (Kairo).
From: Sustainable and portable CRISPR-based diagnostics for high-sensitivity Mpox detection
A Set-up diagram illustrating the use of a hand warmer (Kairo) as an alternative and disposable heat source for the RPA and CONAN assay. B Collateral cleavage activity of EMX1-targeting CRISPR-Cas3 after RPA amplification with incubation using a thermal cycler or a hand warmer. ns: no significance. The template was 10 fM human genomic DNA derived from HEK293T cells. Statistical significance was tested using a two-way ANOVA with Sidak’s post-hoc test. C Collateral cleavage activity and lateral flow detection of CRISPR-Cas3 after the CONAN reaction with hand-warmer incubation for 10 min. RPA products from B were used. #: saturated signals on the CFX Connect device. Positive (red arrow) and negative (black arrow) bands for CONAN. D Kairo-RPA and Kairo-CONAN detecting MPXV oligo DNA. RPA was performed with 10 fM of MPXV_CDC oligo DNA for 40 min and the RPA product was detected by the CONAN reaction for 10 min. All incubations were performed using a Kairo.
