Fig. 1: Investigation of different types of activation triggers for Cas13a RNP.
From: Autocatalytic Cas13a biosensor enabled by RNA-nanocircles for ultrasensitive RNA detection
A Schematic of a single strand or double strand trigger of Cas13a RNP; (B) Investigation of different types of single strand trigger, including single strand RNA (ssRNA), phosphorothioate-modified single strand RNA (ps-ssRNA) and single strand DNA (ssDNA) (n = 3); (C) Investigation of different types of double strand trigger, including double strand RNA (ssRNA-cRNA), RNA-DNA hybrid trigger (ssRNA-cDNA), double strand DNA (ssDNA-cDNA), and ps-ssRNA-cDNA hybrid trigger (ps-ssRNA-cDNA) (n = 3); (D) Schematic of the FRET approach for the investigation of the dsRNA Cas13a activation mechanism. t-RNA represents target RNA, c-RNA represents complementary RNA; (E) FRET measurements shed light on the dsRNA Cas13a activation mechanism confirming the binding of t-RNA on the gRNA of Cas13a (n = 3). (*P < 0.05, **P < 0.005, ***P < 0.001).
