Fig. 3: Calibration curves for the five viral NPs in nasopharyngeal swab:lysis buffer (1:1).

Chips are biofunctionalized as follows: CH1: anti-IBV; CH2: anti-RSV; CH3: anti-IAV; CH4: anti-COV. Individual NP solutions (ranging from 100 to 5000 ng/mL) were prepared and flowed across all the channels. Calibration curves for each specific target/channel are shown: A NP from Influenza B, B NP from RSV A (solid line) and B (dashed line (---)), C NP from Influenza A, D NP from SARS-CoV-2. The non-specific signals from the non-target nucleoproteins in each channel are also shown. Each data point represents the mean ± SD of duplicate measurements. The dotted line (···) indicates the zero value for reference.