Table 2 Recovery study with blind spiked samples with the multiplexed plasmonic biosensor

Samples	Spiked NP		Measured NP					Recovery (%)
	Virus	[NP] (ng/mL)	CH1 Anti-IBV	CH2 Anti-RSV	CH3 Anti-IAV	CH4 Anti-COV	[NP] (ng/mL)
	Virus	[NP] (ng/mL)	Δλ_CH1 (nm)	Δλ_CH2 (nm)	Δλ_CH3 (nm)	Δλ_CH4 (nm)	[NP] (ng/mL)
S1	Influenza B	4000	(+) 2.90	(-) 0.02	(-) 0.00	(-) 0.04	3729.7	93.2
S2	Influenza B	1000	(+) 1.01	(-) 0.03	(-) 0.02	(-) 0.00	1069.4	106.9
S3	Influenza A	500	(-) 0.03	(-) 0.01	(+) 0.23	(-) 0.01	791.4	158.3
S4	SARS-CoV-2	1800	(-) 0.03	(-) 0.04	(-) 0.04	(+) 2.69	2689.8	149.9
S5	RSV B	10	(-) 0.02	(-) 0.00	(-) 0.03	(-) 0.02	< LOD	-
S6	Influenza A	3500	(-) 0.03	(-) 0.00	(+) 0.70	(-) 0.03	3312.4	94.6
S7	Influenza A	1200	(-) 0.02	(-) 0.02	(+) 0.40	(-) 0.02	1700.9	141.7
S8	Influenza B	100	(+) 0.13	(-) 0.02	(-) 0.03	(-) 0.04	128.7	128.7
S9	SARS-CoV-2	50	(-) 0.03	(-) 0.03	(-) 0.03	(+) 0.15	46.62	93.2
S10	Influenza B	1500	(+) 1.25	(-) 0.00	(-) 0.00	(+) 1.69	1353.2	90.2
S10	SARS-CoV-2	800	(+) 1.25	(-) 0.00	(-) 0.00	(+) 1.69	866.9	108.3
S11	Influenza A	2000	(-) 0.04	(-) 0.02	(+) 0.41	(+) 0.76	1700.9	85.1
S11	SARS-CoV-2	400	(-) 0.04	(-) 0.02	(+) 0.41	(+) 0.76	269.1	67.2

^*Sample was considered positive (+) or negative (-) for a given virus for signals above the set threshold (Δλ < 0.059 nm) or below (Δλ < 0.058 nm). Samples in between those values would be considered “indetermined”. Determination of the cut-off values is described in the Experimental section.

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