Fig. 4: The impact of high-fiber diets on taurine and expression of BA- and taurine-related factors. | npj Gut and Liver

Fig. 4: The impact of high-fiber diets on taurine and expression of BA- and taurine-related factors.

From: Comparative analysis of dietary fiber impact on bile acid metabolism and gut microbiota composition in mice

Fig. 4

The levels of taurine conjugates (A, B), free taurine (C, D), and taurine-glutathione (GSH) conjugate (E) were assessed in the liver (A, C) and ileum mucosa (B, D, and E) using HPLC-MS/MS. Gene expression was measured in the liver (F) and the ileum mucosa (H) with qRT-PCR. Genes: Bal: bile acid CoA ligase; Bsep: bile salt export pump; Cdo: cysteine dioxygenase; Cyp8b1: sterol 12-α-hydroxylase; Cyp27a1: sterol-27-hydroxylase; Fgf15: fibroblast growth factor 15; Fxr: farnesoid X receptor; Ibat: ileal bile acid transporter; Mgst1: Microsomal Glutathione S-Transferase 1; Ntcp: Na + /taurocholate cotransporting polypeptide; Ostα: organic solute transporter α; Shp: small heterodimer partner; Slc6a6: Solute Carrier Family 6 Member 6 (taurine transporter); Tgr5: takeda G protein-coupled receptor 5. CYP7A1 (cholesterol 7-α-hydroxylase) and β-Actin protein levels were verified with western blot and quantified using ImageJ software (G). ANOVA was applied to assess statistical differences between the groups. * represents statistical significance, # indicates a trend (0.0055 < p < 0.05); n = 4–5. Error bars stand for ±SEM.

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