Fig. 2: Duodenal tryptase release is increased in FD despite similar mast cell numbers. | npj Gut and Liver

Fig. 2: Duodenal tryptase release is increased in FD despite similar mast cell numbers.

From: Limited duodenal immune activation in functional dyspepsia is not linked to spinal sensory neuron activation

Fig. 2

A Experimental workflow of eosinophil and mast cell quantification and mediator release. Created with BioRender.com. B Tryptase release from duodenal biopsy supernatants (24 h incubation) measured by ELISA and normalized to biopsy weight. Flow cytometric quantification of duodenal lamina propria (C) mast cells (CD117+ Siglec-8+) and (D) eosinophils (CD193+ Siglec-8+ SSC+). E Eosinophil-derived neurotoxin (EDN) release from duodenal biopsy supernatants (24 h incubation) measured by ELISA and normalized to biopsy weight. Median fluorescence intensity (MFI) within the eosinophil gate of (F) CD125 (IL-5Rα) and (G) CD69. Graphs depict mean ± standard deviation with data points corresponding to individual participants.

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