Table 1 Characterization of aspartic proteases (APs) in Solanum lycopersicum and comparative analysis with known APs from other species

From: Tomato aspartic proteinases harbouring PSI domains reveal stress responsiveness, organ specificity, and conserved features

Species

Name

Accession number

PSI

PSI glycosylation motif

ctVSD

C. cardunculus

Cardosin A

AJ132884

✔

⨉

VGFAEAA

Cardosin B

AJ237674

✔

NETE

VGFAEAV

A. thaliana

AtAP A1

AT1G11910

✔

NMTQ

VGFAEAA

H. vulgare

Phytepsin

P42210

✔

NMTQ

IGFAKAA

S. tuberosum

StAP

AY672651

✔

⨉

VGFAEVHNSL

G. max

SoyAP1

NM_001250416

✔

NETE

VGFADAA

SoyAP2

NM_001250135

✔

⨉

VGFAEAV

C. vulgare

Cirsin

JN703462

✔

NETE

VGFAEAA

C. reinhardtii

Chlapsin

AJ579366

✔

NSTI

LGFANAA

S. lycopersicum

-

Solyc00g005000

⨉

-

⨉

-

Solyc01g081350.1.1

⨉

-

⨉

-

Solyc01g081370.2.1

⨉

-

⨉

-

Solyc01g096040.2.1

⨉

-

⨉

-

Solyc01g111850.2.1

⨉

-

⨉

-

Solyc02g065050.1.1

⨉

-

⨉

-

Solyc05g009680.1.1

⨉

-

⨉

-

Solyc05g016310.1.1

⨉

-

⨉

-

Solyc05g051240.1.1

⨉

-

⨉

-

Solyc05g056450.2.1

⨉

-

⨉

-

Solyc06g069220.1.1

⨉

-

⨉

-

Solyc07g006430.1.1

⨉

-

⨉

-

Solyc07g006440.1.1

⨉

-

⨉

-

Solyc07g006450.1.1

⨉

-

⨉

-

Solyc07g006460.1.1

⨉

-

⨉

-

Solyc07g006470.1.1

⨉

-

⨉

-

Solyc07g045100.1.1

⨉

-

⨉

-

Solyc08g005820.1.1

⨉

-

⨉

-

Solyc08g005830.1.1

⨉

-

⨉

-

Solyc08g005840.1.1

⨉

-

⨉

-

Solyc08g005850.1.1

⨉

-

⨉

-

Solyc08g074920.1.1

⨉

-

⨉

-

Solyc08g074930.1.1

⨉

-

⨉

-

Solyc09g011040.1.1

⨉

-

⨉

-

Solyc10g009410.1.1

⨉

-

⨉

-

Solyc06g009080.1.1

⨉

-

⨉

-

Solyc06g009090.1.1

⨉

-

⨉

-

Solyc06g009110.2.1

⨉

-

⨉

-

Solyc06g018100.2.1

⨉

-

⨉

-

Solyc06g060130.2.1

⨉

-

⨉

-

Solyc06g069190.2.1

⨉

-

⨉

-

Solyc01g096450

⨉

-

⨉

AP Y

Solyc02g032940.2.1

✔

NQT

VGFAEAA

-

Solyc02g083450.2.1

⨉

-

⨉

AP V

Solyc03g005280.2.1

✔

NGT

VGFAESA

-

Solyc03g033360.1.1

⨉

-

⨉

-

Solyc03g117690.2.1

⨉

-

⨉

-

Solyc03g119690.1.1

⨉

-

⨉

-

Solyc04g008850.1.1

⨉

-

⨉

-

Solyc04g076190.1.1

⨉

-

⨉

-

Solyc05g053900.2.1

⨉

-

⨉

-

Solyc06g068550.2.1

⨉

-

⨉

-

Solyc06g074000.1.1

⨉

-

⨉

AP W

Solyc07g051850.2.1

✔

NETA

VGFAEAA

-

Solyc07g054650.1.1

⨉

-

⨉

-

Solyc08g067100.2.1

⨉

-

⨉

-

Solyc08g068860.2.1

⨉

-

⨉

-

Solyc08g068870.2.1

⨉

-

⨉

-

Solyc08g074940.2.1

⨉

-

⨉

-

Solyc09g059750.1.1

⨉

-

⨉

AP X

Solyc09g082760.2.1

✔

NET

IGFAESA

-

Solyc09g098340.2.1

⨉

-

⨉

-

Solyc10g005890.2.1

⨉

-

⨉

-

Solyc11g011440.1.1

⨉

-

⨉

-

Solyc11g068880.1.1

⨉

-

⨉

-

Solyc12g087940.1.1

⨉

-

⨉

-

Solyc02g069360.1.1

⨉

-

⨉

AP Z

Solyc01g101240.2.1

✔

⨉

VGFAEAA

  1. A total of 58 APs were identified in S. lycopersicum using the Solgenomics database, five of which contained plant-specific inserts (PSIs): AP X, AP W, AP X, AP Y, and AP Z. All PSI-containing APs also contained a ctVSD sequence and were further analyzed for glycosylation motifs on PSIs. Specifically, PSI V, PSI W, PSI X, and PSI Y contained the glycosylation motifs NGT, NETA, NET, and NQT, respectively, while PSI Z lacked a glycosylation motif. These sequences were also compared with known APs from other species, including C. cardunculus (cardosin A and cardosin B), A. thaliana (AtAP A1), H. vulgare (phytepsin), S. tuberosum (StAP), G. max (soyAP1 and soyAP2), C. vulgare (cirsin), and C. reinhardtii (chlapsin).
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