Table 2 MSC therapies for AAA models
Disease model | Cell type | Delivery Method | Day(s) of MSC Delivery | Day(s) of Harvest | Output Measurement | Reference |
|---|---|---|---|---|---|---|
Angiotensin II in ApoE mice | Murine BM-MSC | Multiple IV injections | Days 0, 7, 14, 21 | Day 28 | Decreased aortic diameter in infrarenal regions; elastin content of MSC group significantly higher than control, also suppressed inflammatory response and MMP activity | |
Elastase model in mice | Human umbilical cord-MSC | IV injection | Day 0 | Day 14 | MSC anti-HMGB1 treatment attenuated AAA formation IL-17 production and inflammatory infiltration | |
Collagenase and elastase model in pigs | Porcine BM-MSC | Direct injection into aortic wall | Day 1 | Days 1, 3, 7 | Expressed high levels of VEGF in MSC group | |
Elastase model in mice | Murine BM-MSC | IV injection | Days 1, 3, 5 | Day 14 | Both Male and Female MSC decreased aortic dilation TNF-α, IL-1β, and MCP-1 were only decreased with female MSC | |
Angiotensin II in ApoE mice | Murine BM-MSC | Cell sheets | Day 0 | Day 28 | MSCs decreased active MMP-2, preserved elastin content and AA development was site specifically inhibited | |
Angiotensin II in ApoE mice | Murine BM-MSC | IV injection | Day 28 | Days 42, 56, 84 | No significant difference at 8 weeks for aortic diameter. Pro and active mmp2/9 were significantly reduced in MSC groups at week 2, elastin in the media was preserved at all three timepoints. No statistical difference between IL-1β, TNF-α, TGF-β1 | |
Guinea pig xenograft in rat | Rat BM-MSC | Direct injection to aneurysm | Day 14 | Days 21, 42 | MSCs decreased AAA expansion compared to culture medium infusion, decreased expression of MMP-9 and macrophage infiltration | |
Elastase-induced rat model | Human UC-MSCs | IV injection | Day 0 | Day 14 | The treated group demonstrated effectively decreased AAA expansion, with increased elastin preservation and contractile VSMC marker expression and decreased pro and active MMP2 and MMP9 expression and TNFα | |
Calcium chloride and elastase model in mice | Multilineage-differentiating stress-enduring cells isolated from human BM-MSCs | Multiple IV injections | Days 0 (3 days post aneurysm induction), 7, 14 | Days 21, 56 | At 8 weeks no difference between single injection vs multiple injections of muse cells; both significantly smaller diameters than the non-muse vehicle and MSC groups; multiple injection group had a higher ratio of elastin content than the non-muse vehicle and MSC groups; all groups had significantly lower F4/80+ cells than the vehicle |
