Fig. 1: Intramyocardial injections components and key developments.
a Intramyocardial injections can be single cell solutions or 3D microtissues. b Inspired by a figure of a large hPSC-CM engraftment by Liu et al.41. (Top) control heart. (Bottom) hPSC-CM treated heart. The blue regions represent collagen 1, the pink is cTnT, and the green is human cTnI. c Fukuda et al. specialized delivery system. (Top left) spheroids in phase contrast42. (Top right) schematic of spheroid distribution upon injection42. (Bottom) distribution of tissue marking dye delivered through the injection device (left) long axis (right) short axis42. d Nanowired organoid by Tan et al. (Top left) stained for alpha sarcomeric actinin (green), Vimentin (red) nanowires (yellow) and DAPI (blue) (top right) stained for alpha sarcomeric actinin (green), nanowires (yellow) and DAPI (blue) (Bottom left) stained for alpha sarcomeric actinin (green), Vimentin (red) and DAPI (blue) (bottom right) stained for alpha sarcomeric actinin (green), von Willebrand Factor (red) and DAPI (blue)9. c Reprinted from ref. 42 Copyright (2019), with permission from Elsevier. d From Tan et al. Nanowired human cardiac organoid transplantation enables highly efficient and effective recovery of infarcted hearts. Science Advances 9, eadf2898 https://doi.org/10.1126/sciadv.adf2898. Reprinted with permission from AAAS.
