Fig. 8: PGRN deficiency does not lead to an obvious alteration in myelination and behavior of hTDP-43Tg/+ mice.
From: Progranulin deficiency does not exacerbate TDP-43 pathology in TDP-43 transgenic mouse models
A, B Western blot analysis of the myelin markers in the brain. The levels of MAG, MBP, and PLP in 21-day-old WT, hTDP-43Tg/Tg, Grn−/−, and hTDP-43Tg/Tg Grn−/− mice were analyzed by western blot. The levels were quantified by ImageJ and normalized to the loading control GAPDH. Data are presented as means ± SEM (n = 3–7). p-values were determined using one-way ANOVA tests with Bonferroni’s multiple comparisons. C, D The hindlimb clasping test was performed on 6-month-old WT, hTDP-43Tg/+(Tg/+), Grn−/−, and hTDP-43Tg/+ Grn−/− (Tg/+ Grn−/−) male and female mice. The hindlimb severity score was quantified. Data are presented as means ± SEM (n = 8–12). One-way ANOVA tests. E Six-month-old male and female mice were subjected to footprint tests. The hindlimb stride length was measured. Data are presented as means± SEM (n = 8–12). p-values were determined using one-way ANOVA tests with Bonferroni’s multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001.
