Fig. 2: Evaluation of TREM2 expression in transfected cell models.
From: A novel TREM2 L15Q in signal peptide domain and its influence on Aβ pathogenicity
A RT-qPCR analysis of TREM2 gene expression in MOCK and transfected cells (n = 3). Increased expression confirms successful transfection. B TREM2 protein levels in the solubilized membrane, measured by commercial ELISA (n = 6) with increasing protein levels. C Representative Western blot of TREM2 protein forms in cell lysate, including glycosylation-dependent changes. Treatment with Endo H and PNGase F was used to distinguish mature and immature forms of TREM2 based on glycosylation status. The immature, ER-retained form (lower band) is sensitive to Endo H, while the mature, fully glycosylated form (upper band) is resistant to Endo H but sensitive to pNGase F. Band intensities were quantified using ImageJ and normalized to ACTB. Mature-to-immature ratio was calculated under the control group relative to WT and was decreased in L15Q cells (n = 6). D Representative Western blot of soluble TREM2 (sTREM2) in the cell medium through immunoprecipitation. sTREM2 band intensities were quantified using ImageJ and normalized to WT (n = 4). All Data represent mean ± SD. P-value was obtained using one-way ANOVA followed by Bonferroni’s post hoc test with significance set at P < 0.05. * P < 0.05; ** P < 0.01; **** P < 0.0001.
