Fig. 1: APPE590D increases Aβ and Aη generation.
From: APP E590D mutation increases generation of Aβ and Aη peptides and exacerbates tauopathy
A Schematic of APP770, APP695, and sAPPβ with corresponding domains, cleavage sites (η, β, & α), mutation site (E665D or E590D), and antibody epitopes. B Representative blots of HEK293T cells transfected with vector control (VC), APP695WT, or APP695E590D and subjected to Western blotting from the medium and lysates with D54D2 antibody (medium : Aη & Aβ), sAPPβ antibody (medium), 6E10 antibody (lysate: APP, CTFη, & CTFβ), and actin antibody (lysate). Quantification of (C) Aβ, (D) CTFβ, (E) sAPPβ, (F) Aη and (G) CTFη levels normalized to total APP expression (t test, **P < 0.01, ****P < 0.0001, n = 6–9 / group). H Blots of mouse primary neurons transduced with rAAV9 expressing vector control (VC), APP695WT, or APP695E590D at DIV14 for 7 days and subjected to Western blotting from the medium and lysates with D54D2 antibody (medium : Aη & Aβ), sAPPβ antibody (medium), 6E10 antibody (lysate: APP), and actin antibody (lysate). Quantification of (I) Aβ, (J) sAPPβ, and (K) Aη levels normalized to total APP expression (t-test, *P < 0.05, **P < 0.01, n = 3 / group).
