Fig. 3: APPE590D increases tau seeding and aggregation.
From: APP E590D mutation increases generation of Aβ and Aη peptides and exacerbates tauopathy
A Representative images of Tau-RD biosensor cells transfected with vector control (VC), APP695WT, or APP695E590D for 24 h and subjected to treatment with sonicated tau seeds from 8-month-old tauP301S mouse brain for 24 h. Representative images showing tau-RD puncta (green) and APP staining (red, 6E10). Yellow arrows showing tau-RD puncta (green) in APP695E590D cells (red). B Quantification of tau-RD puncta intensity normalized by APP expression (red) (1-way ANOVA, post hoc Tukey, *P < 0.05, **P < 0.001, ns=not significant, n = 3, 20 images/replicate). C–F Hela-V5-tau cells stably expressing 4R tau transfected with vector control (VC), APP695WT, or APP695E590D for 48 h and subjected to (C) Western blotting for APP (6E10), tau (A-10), and actin from the RIPA-soluble fraction (1-way ANOVA, post hoc Tukey, *P < 0.05, n = 3 / group); (D) quantification of soluble tau from the RIPA-soluble fraction and (E) filter-trap assay from the RIPA-insoluble fraction to capture tau aggregates. F Quantification of tau aggregates captured by filter-trap assay from the RIPA-insoluble fraction (1-way ANOVA, post hoc Tukey, **P < 0.01, ***P < 0.001, n = 3 / group).
