Fig. 4: APP695E590D exacerbates tauopathy and neuroinflammation in tauP301S mice.
From: APP E590D mutation increases generation of Aβ and Aη peptides and exacerbates tauopathy
A–C TauP301S mice (5 mo) transduced with purified high-titer APP695E590D rAAV9, APP695WT, or control by stereotaxic injection into the hippocampus for 3 months. Equal amounts of RIPA soluble and insoluble protein lysates from the hippocampus subjected to immunoblotting for APP (6E10), phospho-tau (pS199/202), and actin. B–D Quantification of soluble and insoluble phospho-tau (pS199/202), and Aη (1-way ANOVA, post hoc Tukey, *P < 0.05, ** P < 0.01, n = 3 mice for control and APP695E590D groups (one mouse died from each group after injections), n = 4 mice for APP695WT). E Representative images of brain slices from the hippocampus and cortex processed for confocal microscopy after IHC for phospho-tau (pS199/202, green). F, G Quantification of phospho-tau (pS199/202) from the cortex and hippocampus (1-way ANOVA, post hoc Tukey, *P < 0.05, ** P < 0.01, n = 3 mice/group, 15–19 images/mouse). H Representative hippocampal images of brain slices processed for confocal microscopy after IHC for IBA1 (green), and GFAP (red). I, J Quantification of hippocampal IBA1 and GFAP intensity (1-way ANOVA, post hoc Tukey, *P < 0.05, ** P < 0.01, n = 3 mice/group, 11–12 images/group).
