Table 4 The elution gradient program of UPLC-MS/MS
Time(min) | Methanol(%) | Ammonium acetate solution(%) |
|---|---|---|
0 | 30 | 70 |
5 | 65 | 35 |
9 | 95 | 5 |
11 | 95 | 5 |
11. 1 | 30 | 70 |
12 | 30 | 70 |
Time(min) | Methanol(%) | Ammonium acetate solution(%) |
|---|---|---|
0 | 30 | 70 |
5 | 65 | 35 |
9 | 95 | 5 |
11 | 95 | 5 |
11. 1 | 30 | 70 |
12 | 30 | 70 |