Figure 4: Gene fold change, and localisation of target protein expression in cornea sections.

(a) is the gene fold change by microarray. (b) is the gene fold change by qPCR. n=4 and 3 corneas for (a,b), respectively. ANOVA followed by Tukey’s multiple comparisons test was used to isolate pairwise differences, with alpha <0.05. In both (a,b), asterisks represent adjusted P<0.05 and the error bars represent standard deviation. ns, not significant. The data in (a,b) was analysed using GraphPad Prism 5 (GraphPad Software, Inc. CA 92037 USA). (c) is the localisation of the corresponding protein expression in cornea cross sections stained by immunohistochemistry. White scale bars represent 20 μm.