Figure 6
From: Connexin43 Functions as a Novel Interacting Partner of Heat Shock Cognate Protein 70
Cx43 Prevents the Nuclear Translocation of Hsc70.
(a) Hsc70 translocated into the nucleus under heat shock. HuH-7 cells were heat shocked at 42°C for 2 hr and stained with anti-Hsc70 antibodies. (b) Overexpression of Cx43 prevented the nuclear translocation of Hsc70 under heat shock. HuH-7 cells were transfected with control vector EGFP or Cx43-EGFP. Forty-eight hours after transfection followed by heat shocked at 42°C for 2 hr, cells were stained with anti-Hsc70 antibodies. (c) Ratio of nuclear/cytoplasmic (N/C) fluorescence intensities in Hsc70. After heat shock, at least 30 transfected HuH-7 cells of control EGFP or Cx43-EGFP were examined and quantified by confocal scanning microscopy. The data shown in (b) were plotted. The horizontal lines represent mean values. *p < 0.01.
