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Showing 1–6 of 6 results
Advanced filters: Author: Akihiro Kishimura Clear advanced filters

  • Polyion complex (PIC) formation is one of the most powerful techniques for obtaining molecular self-assemblies in aqueous media, and useful for material syntheses particularly in the biomedical field. In this review article, recent progress on PIC vesicles (PICsomes) is summarized. PICsomes are characterized by their simple preparation, semipermeability and environment-sensitivity. Very recently, the methods to control structural uniformity and size of the vesicles have been established, and the potential utility of PICsomes has been expanded by crosslinking their PIC layers. In addition, the unique dynamic nature of PICs is also described.

    • Akihiro Kishimura
    Reviews
    Polymer Journal
    Volume: 45, P: 892-897

  • A thermoresponsive coacervate enabling the sustained release of micelles and siRNA was developed using an appropriate blend of ABA/AB block copolymers. At room temperature, siRNA-loaded polyion complex (PIC) micelles were dispersed in solution. Upon mild heating ( ≈ 45 °C), they assembled into coacervate microparticles through a reversible phase transition. When maintained at 37 °C, the coacervates continuously and constantly release siRNA and micelles. Biological activities were evaluated using living cells, and cellular uptake and gene knockdown effects were confirmed. This concept highlights a coacervate-based depot platform for siRNA delivery system.

    • Ziwei Ma
    • Hiroshi Kamizawa
    • Akihiro Kishimura
    Research
    Polymer Journal
    P: 1-12

  • The aim of this study is to establish the factors that affect the formation of polyion complex (PIC) vesicles. The effects of anionic segment length of PEG-PAspy, and chemical structure of the side chains of Homo-P(Asp-Cn)x on the properties of PICs were carefully examined. It was found that PEG weight fraction (fPEG) has a significant role in determining the PIC morphologies as PICs with fPEG <10% formed submicron/nanosized polyion complex vesicles (Nano-PICsomes), whereas micelles were selectively fabricated at fPEG >10%. In addition, Homo-P(Asp-Cn)x containing five and six carbon atoms on the aliphatic spacers in their side chains facilitated the formation of Nano-PICsomes (compared with the shorter ones). These findings shed new light on how to control the structure and properties of PIC assemblies for diverse material applications.

    • Sayan Chuanoi
    • Akihiro Kishimura
    • Kazunori Kataoka
    Research
    Polymer Journal
    Volume: 46, P: 130-135

  • Complex coacervates (CCs) are a promising platform for protein encapsulation obtained via simple mixing of some components. Using the novel precoating treatment of living cells, the cellular internalization of CCs was greatly enhanced. Besides, upon using the side-chain modified aniomers for CC fabrication, a wide variety of proteins were successfully encapsulated, which can expand the future biomedical application of CCs.

    • Bing Jhang Li
    • Biplab K C
    • Akihiro Kishimura
    Research
    Polymer Journal
    Volume: 57, P: 911-922