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Showing 1–12 of 12 results
Advanced filters: Author: Ashty S. Karim Clear advanced filters

  • Cost-effective, environmentally sustainable and energy-efficient ways to address rising atmospheric CO2 levels are urgently needed. Here the authors combine electrochemical reduction of CO2 to formate with biosynthetic conversion of formate to the universal building block acetyl-CoA using a synthetic metabolic pathway called ReForm.

    • Grant M. Landwehr
    • Bastian Vogeli
    • Michael C. Jewett
    Research
    Nature Chemical Engineering
    Volume: 3, P: 57-69

  • Allosteric transcription factors (aTFs) are promising tools for environmental and human health monitoring. Here the authors develop a multi-objective, machine learning-guided method to engineer an aTF-based portable diagnostic for environment sensing of lead in drinking water at the legal limit.

    • Brenda M. Wang
    • Nicole Chiang
    • Michael C. Jewett
    ResearchOpen Access
    Nature Communications
    Volume: 17, P: 1-14

  • Post-translational modifications (PTMs) are important for the stability and function of many therapeutic proteins. Here, the authors develop a high-throughput workflow combining cell-free gene expression with AlphaLISA to rapidly characterize and engineer PTMs on both proteins and peptides.

    • Derek A. Wong
    • Zachary M. Shaver
    • Michael C. Jewett
    ResearchOpen Access
    Nature Communications
    Volume: 16, P: 1-18

  • While machine learning shows promise in expanding protein engineering efforts, its potential is limited by the challenge of gathering large datasets of sequence-function relationships. Here, authors introduce a platform that integrates cell-free DNA assembly and gene expression to accelerate enzyme engineering.

    • Grant M. Landwehr
    • Jonathan W. Bogart
    • Michael C. Jewett
    ResearchOpen Access
    Nature Communications
    Volume: 16, P: 1-13

  • Model-based part design is a key step in synthetic biology. Here, the authors report a method for tuning nucleosome architecture in order to strengthen native promoters and facilitate synthetic promoter design in yeast.

    • Kathleen A. Curran
    • Nathan C. Crook
    • Hal S. Alper
    Research
    Nature Communications
    Volume: 5, P: 1-8

  • An attractive route for carbon-negative synthesis of biochemical products is the reverse β-oxidation pathway coupled to the Wood-Ljungdahl pathway. Here the authors use a high-throughput in vitro prototyping workflow to screen 762 unique pathway combinations using cell-free extracts tailored for r-BOX to identify enzyme sets for enhanced product selectivity.

    • Bastian Vögeli
    • Luca Schulz
    • Michael C. Jewett
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-10

  • Antibody discovery is bottlenecked by the individual expression and evaluation of antigen specific hits. Here, the authors build an antibody screening workflow leveraging cell-free protein synthesis that enables expression and evaluation of hundreds of antibody fragments in less than 24 h.

    • Andrew C. Hunt
    • Bastian Vögeli
    • Michael C. Jewett
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-14

  • Developing biotechnologies to address society’s challenges requires integrating concepts across disciplines, posing challenges to educating students with diverse expertise. In this Perspective the authors create a framework for synthetic biology training that deconstructs biotechnologies across spatial scales.

    • Ashty S. Karim
    • Dylan M. Brown
    • Julius B. Lucks
    ReviewsOpen Access
    Nature Communications
    Volume: 15, P: 1-14

  • The iPROBE platform accelerates the design and optimization of engineered biosynthetic pathways using a combination of cell-free protein synthesis, in vitro pathway assembly and a scoring system to identify high-performing combinations.

    • Ashty S. Karim
    • Quentin M. Dudley
    • Michael C. Jewett
    Research
    Nature Chemical Biology
    Volume: 16, P: 912-919

  • Cell-free gene expression systems have long been used to address fundamental research questions. Now, owing to technological advances, these systems are finding wider applications in the field of synthetic biology, including in biosensing, biomanufacturing, education and the design of gene networks.

    • Adam D. Silverman
    • Ashty S. Karim
    • Michael C. Jewett
    Reviews
    Nature Reviews Genetics
    Volume: 21, P: 151-170