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Showing 1–4 of 4 results

Advanced filters: Author: Brad Nelms Clear advanced filters

FX-Cell: a method for single-cell RNA sequencing on difficult-to-digest and cryopreserved plant samples

This work describes FX-Cell and its derivatives to achieve single-cell RNA sequencing from challenging and cryopreserved plant tissues, expanding the scope of single-cell genomics across field-grown samples.

Xin Ming
Mu-Chun Wan
Jia-Wei Wang
Research27 Nov 2025
Nature Methods

Volume: 22, P: 2551-2562
Potent pollen gene regulation by DNA glycosylases in maize

DNA glycosylases license expression of a set of pollen-specific, highly expressed maize genes that are of interest not only because of their transposon-like DNA methylation but also because of their predicted importance in cell wall modification.

Yibing Zeng
Julian Somers
Jonathan I. Gent
ResearchOpen Access27 Sept 2024
Nature Communications

Volume: 15, P: 1-12
The peri-germ cell membrane: poorly characterized but key interface for plant reproduction

Naoya Sugi
Andrea R. M. Calhau
Thomas Widiez
Correspondence15 Oct 2024
Nature Plants

Volume: 10, P: 1607-1609
Single-cell RNA-seq of maize meiocytes and pollen grains

The authors provide a protocol to microscopically identify and isolate single maize meiocytes and pollen grains for RNA-seq with a modified version of CEL-Seq2. Staging of developmentally synchronized anthers is performed in parallel with expression analysis.

Mary Washburn
Jaime Alaniz-Fabián
Brad Nelms
Protocols02 Oct 2023
Nature Protocols

Volume: 18, P: 3512-3533