Class-A β-lactamases can render antibiotics ineffective through hydrolysis, but the associated enzyme-antibiotic complexes remain largely underexplored at room temperatures. Here, the authors use RT serial synchrotron crystallography to report acyl-enzyme intermediates of the catalytically impaired β-lactamase CTX-M-14 mutant (E166A) from Klebsiella pneumoniae, and show that different isoxazolyl-penicillins adopt different conformations at room temperature.
- Gargi Gore
- Andreas Prester
- Eike C. Schulz
