Native chemical ligation is pivotal in chemical protein synthesis but often requires excessive additives due to the low reactivity of peptide alkyl thioesters. Here, the authors introduce a thioacid-based additive-free peptide ligation using vinyl thianthrenium tetrafluoroborate, enhancing efficiency and simplicity in synthesizing complex proteins like hyalomin-3 and ubiquitin.
- Hanghang Li
- Shujuan Xu
- Jiaan Liu
