The ESCRT complex mediates sorting of ubiquitylated endosome-associated proteins into multivesicular bodies (MVBs). The RNA-induced silencing complex (RISC) components GW182 and AGO2 localize to membrane structures that congregate with MVBs. Loss of ESCRT function compromises miRNA-mediated silencing and increases GW182 levels, suggesting that ESCRT regulates RNAi by acting on GW182 turnover.
- Derrick J. Gibbings
- Constance Ciaudo
- Olivier Voinnet
