Heavy water metabolic labeling followed by liquid chromatography coupled mass spectrometry (LC-MS) is a powerful approach to characterize in vivo protein turnover rates, however, peptide co-elution causes overlap of their isotope profiles in LC-MS and affects the proteome coverage. Here, the authors develop an approach to increase the proteome coverage for in vivo protein turnover by using partial isotope profiles from two mass isotopomers.
- Henock M. Deberneh
- Doaa R. Abdelrahman
- Rovshan G. Sadygov
