It is difficult to apply SMLM to complex biological tissues. Here the authors report REALM, Robust and Effective Adaptive Optics in Localisation Microscopy, to improve SMLM in tissue and use this to resolve the organisation of spectrin in the axon initial segment in brain tissue.
- Marijn E. Siemons
- Naomi A. K. Hanemaaijer
- Lukas C. Kapitein