Targeting RNA with small molecules represents an attractive medicinal approach, and profiling the small molecule-binding landscapes of various types of RNA structures is critical. Here, the authors develop a large-scale approach for investigating small molecule-RNA structure interaction profiles using a multiplexed pull-down assay with various RNA structure libraries, and validate the assay using G-clamp and thiazole orange derivatives.
- Ryosuke Nagasawa
- Kazumitsu Onizuka
- Fumi Nagatsugi
