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Double-helix optical point spread function enables real-time mesoscopic 3D functional microangiography in the living mouse brain and skull

A mesoscopic fluorescence imaging platform incorporating a double-helix phase mask enables realtime 3D mapping of cerebrovascular networks and blood flow dynamics in the living mouse brain under healthy conditions and in glioma.

Baoyuan Zhang
Shiyao Guo
Quanyu Zhou
ResearchOpen Access13 Apr 2026
Nature Communications

P: 1-15

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Double-helix optical point spread function enables real-time mesoscopic 3D functional microangiography in the living mouse brain and skull

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