This robust, proteome-wide approach for the exploration of the S-nitrosoproteome in human and mouse tissues uses the SNOTRAP probe and nano-liquid chromatography–tandem mass spectrometry analysis. The protocol enables efficient and high-throughput profiling of S-nitrosylated proteins in complex mixtures of biological material.
- Hongmei Yang
- Haitham Amal
- Stuart A. Lipton
