Emerging from DNA/RNA nanoengineering, synthetic nucleic-acid liquid condensates, forming via phase separation of nanostructures, have attracted increasing attention as a powerful platform for synthetic biology and molecular computing. Base-sequence specificity allows for molecular encoding for their organization, functions, and droplet interactions. Authors overview key topics of these programmable droplets, from dynamics programmability to numerical modeling. Additionally, this review highlights cross-linker modules, which enable dynamic compartmentalization and division of droplets triggered by specific molecular input. These modules allow the condensate phase behavior to represent Boolean logic operation.
- Hirotake Udono
- Tomoya Maruyama
- Masahiro Takinoue
