Advanced search

Filter By:

Journal Check one or more journals to show results from those journals only.

Choose more journals

Article type Check one or more article types to show results from those article types only.
Subject Check one or more subjects to show results from those subjects only.
Date Choose a date option to show results from those dates only.

Custom date range

Clear all filters
Sort by:
Showing 1–11 of 11 results
Advanced filters: Author: Jason Micklefield Clear advanced filters

  • The function of putative bacterial vitamin K-dependent carboxylases (VKDCs) has so far been uncertain. Now, Micklefield and co-workers show that a bacterial VKDC orthologue is involved in the biosynthesis of the antibiotic malonomycin, generating an unusual malonic acid moiety that is essential for its biological activity.

    • Brian J. C. Law
    • Ying Zhuo
    • Jason Micklefield
    Research
    Nature Catalysis
    Volume: 1, P: 977-984

  • Biocatalysis and metal catalysis often provide complimentary reactivities and selectivities. Here, the authors exploit the regioselectivity of an enzymatic C–H activation followed by palladium catalysed carbon-carbon bond formation in one pot, with membrane compartmentalization used to isolate the two chemistries.

    • Jonathan Latham
    • Jean-Marc Henry
    • Jason Micklefield
    ResearchOpen Access
    Nature Communications
    Volume: 7, P: 1-8

  • Total in vitro biosynthesis can reveal unusual pathways evolved by nature to produce natural products. Here the authors report on enzymatic cascades, comprising a cryptic methylation sequence, efficiently delivering β-lactone-containing peptide proteasome inhibitors with promising anticancer activity.

    • Guangcai Xu
    • Daniele Torri
    • Jason Micklefield
    ResearchOpen Access
    Nature Chemical Biology
    Volume: 20, P: 1371-1379

  • C−H bond functionalization methodologies usually rely on substrate-controlled directing-group chemistries to facilitate regioselective activation. Now, chemobiocatalytic cascades are reported that enable catalyst-controlled regioselective access to aryl nitriles, primary amides and carboxylic acids.

    • Elliott J. Craven
    • Jonathan Latham
    • Jason Micklefield
    Research
    Nature Catalysis
    Volume: 4, P: 385-394

  • Engineering biosynthetic assembly lines is a powerful path to new natural products but is challenging with current methods. Here the authors use CRISPR-Cas9 to exchange subdomains within NRPS to alter substrate selectivity.

    • Wei Li Thong
    • Yingxin Zhang
    • Jason Micklefield
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-10

  • Proteins, other metabolites and many valuable synthetic products contain amide bonds and there is a need for more sustainable amide synthesis routes. Here the authors show an integrated next generation multi-catalytic system, merging nitrile hydratase enzymes with a Cu-catalysed N-arylation reaction in a single reaction vessel, for the construction of ubiquitous amide bonds.

    • Luis Bering
    • Elliott J. Craven
    • Jason Micklefield
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-10

  • S-adenosylmethionine (SAM)-dependent methyltransferase enzymes have significant synthetic potential, but their utility as biocatalysts has been limited by the availability of SAM. An elegant and simple method addressing this long-standing problem has now been developed using a halide methyltransferase (HMT) enzyme for SAM regeneration in vitro.

    • Jason Micklefield
    News & Views
    Nature Catalysis
    Volume: 2, P: 644-645

  • A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded in biosynthetic gene clusters. Information about these clusters, pathways and metabolites is currently dispersed throughout the literature, making it difficult to exploit. To facilitate consistent and systematic deposition and retrieval of data on biosynthetic gene clusters, we propose the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard.

    • Marnix H Medema
    • Renzo Kottmann
    • Frank Oliver Glöckner
    Comments & OpinionOpen Access
    Nature Chemical Biology
    Volume: 11, P: 625-631

  • Pablo Carbonell et al. present an automated pipeline for the discovery and optimization of biosynthetic pathways for microbial production of fine chemicals. They apply their pipeline to the production of the flavonoid (2S)-pinocembrin in Escherichia coli and show improvement of the pathway by 500-fold.

    • Pablo Carbonell
    • Adrian J. Jervis
    • Nigel S. Scrutton
    ResearchOpen Access
    Communications Biology
    Volume: 1, P: 1-10