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Climate change can alter when and how animals grow, breed, and migrate, but it is unclear whether this allows populations to persist. This global study shows that shifts in seasonal timing are key to helping vertebrate species maintain population growth under global warming.

This study presents BERT, an algorithm for high-performance integration of incomplete omics data with robustness to unequal phenotype distribution. It validates the method on simulated and experimental data from proteomics, metabolomics and transcriptomics.

DNA methylation and copy number variant analyses across a large number of genetic mouse models of pediatric brain tumors reveal subtype-specific molecular alterations shared with the corresponding human diseases.

A comparison of the repertoire of SARS-CoV-2-specific epitopes targeted by T cells induced by vaccination or natural infection reveals that T cells predominantly target non-spike epitopes in convalescent individuals, while there is a broader spike-specific CD8⁺ T-cell response in vaccinees. Despite differences in T-cell response, the targeted T-cell epitopes were conserved between the wild-type and Omicron variants in both groups.

Here the authors identify TNIP1 as a risk factor for a fatal neurodegenerative disorder and discover specific genetic loci associated with the three main subtypes of this disorder. The findings highlight distinct disease mechanisms, emphasizing the roles of immunity and the notch signaling pathway.

Therapy of tuberculosis is challenging, mainly due to complex structures of necrotic granulomas that often impair drug delivery. In this work, the authors show that the drug BTZ-043 fully penetrates necrotic granulomas and has potent lesional antibacterial activity.

A combination of gentle stimulated emission depletion microscopy imaging and deep-learning-based improvements in signal-to-noise ratio enables high-resolution reconstruction of neuronal architecture in living tissue.

Dataset integration is common practice to overcome limitations in statistically underpowered omics datasets. Here the authors present “HarmonizR”, a tool for missing data tolerant experimental variance reduction in large, integrated but independently generated datasets without data imputation, adjustable for individual dataset modalities, correction algorithm, and user preferences.

Mapping fixed brain samples with extracellular labeling and optical microscopy reveals synaptic connections.

Tuft cells constitutively express IL-25 to sustain ILC2 homeostasis in the intestine, but mechanisms driving IL-25 secretion have been unclear. Here, Feng et al. find that tuft cells express IL-17RB, which is required to regulate the bioavailability of IL-25 and to restrain the activation of ILC2s during homeostasis.

Simultaneous activation of Wnt and Shh pathways in murine neural precursor cells results in the formation of embryonal tumors with multilayered rosettes (ETMR) that recapitulate the histological and molecular features of human tumors. This novel mouse model represents a platform for evaluating therapeutic approaches for this rare malignant pediatric brain tumor, and provides novel insights into the cell of origin and molecular mechanisms driving the disease.

A neural epigenetic signature detectable via plasma analyses is prognostic in patients with glioblastoma, resembling an oligodendrocyte-progenitor- and neuronal-progenitor-cell-like state and showing increased neuro-to-glioma synapse formation.

Medulloblastomas (MBs) are highly heterogeneous paediatric brain tumours that remain challenging to treat. Here, the authors integrate proteomics, epigenomics, transcriptomics and post-translational modification analyses to find molecular subgroups and potential therapeutic targets in MB tumours.

A large-scale CRISPR-mediated deep mutational scanning approach is used to interrogate the function of mutations in the endogenous locus of TP53 mapping to the DNA-binding domain.

Vaccines induce beneficial immunity for COVID-19, but immune waning prompts boosting vaccination. Here, the authors show that a third, boosting dose of COVID-19 mRNA vaccine induces transient CD8 + T effector cell response while conserving the CD8 memory T cell pool, thereby permitting reactivation of spike-specific CD8 + T cells upon breakthrough infection or 4th vaccination.

Longitudinal analyses of SARS-CoV-2 mRNA vaccine-elicited epitope-specific CD8⁺ T cell responses shows that CD8⁺ T cells are rapidly induced after prime vaccination and stably maintained after boost vaccination.

It has been shown previously that substrate viscoelasticity affects surface wettability. Here the authors observe a wetting transition during drying of droplets on such substrates and elucidate it with high resolution force field measurements thereby determining its dependence on substrate properties.

Single-cell transcriptomics allows gene expression heterogeneity to be assessed at cellular resolution but the original location of each cell is unknown. Here the authors combine single nuclei RNA-seq with 3D spatial reconstruction of floral meristems to link gene activities with morphology.

This study reports adhibin, a synthetic carbazole that suppresses the migratory and adhesive properties of cancer cells by a mechanism of targeted RhoGAP class-IX myosin inhibition and selective RhoGTPase interference, both translating into migrastatic activity, opening other perspectives in cancer therapy and basic research.

Understanding the various and multiple trade-offs of land-use changes and cropland expansion can contribute to more sustainable policies. A study explores future scenarios of cropland expansion along with the trade-offs in agricultural production and markets, biodiversity and CO₂ emissions.

Paediatric high-grade gliomas with MYCN amplification (HGG-MYCN) are rare and highly aggressive. Here, the authors generate a mouse model for HGG-MYCN that can recapitulate the histological and molecular profiles of the human tumours, and perform high-throughput drug screening to identify potential treatment options.

Injection of engineered bacteria that convert ammonia to l-arginine into tumours enhance the anti-tumour response in a mouse model and synergize with anti-PD-L1 treatment to clear tumours.

The authors show that K-ATP channels in dopamine (DA) neurons of the medial substantia nigra (m-SN) enable burst firing in vitro and in vivo. Silencing K-ATP channel activity in m-SN DA neurons decreases novelty-dependent exploratory behavior in mice.

Data on de novo mutations in Arabidopsis thaliana reveal that mutations do not occur randomly; instead, epigenome-associated mutation bias reduces the occurrence of deleterious mutations.

Severe growth hormone insensitivity syndrome (GHIS) with immunodeficiency is caused by autosomal recessive mutations in STAT5B. Here the authors report heterozygous STAT5B mutations with dominant-negative effects, causing mild GHIS without immune defects.

The synthesis of highly pure diamond nanocrystals with a very small amount of paramagnetic impurities allows the observation of electron spin-dephasing times of up to 1.8 ms, a record for solid-state materials. The result could have important implications for quantum information processing methods based on diamond.

Multiple state-of-the-art analyses of immune cells in 117 blood, 117 cerebrospinal fluid, 13 choroid plexus and 13 brain parenchyma samples reveal differential characteristics of immune cells in different body compartments and different diseases.

Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare inflammatory disorder characterised by asthma, eosinophilia and vasculitis. Here, the authors describe a genome-wide association study of EGPA that reveals clinical and genetic differences between subgroups stratified by autoantibody status (ANCA).

A meta-analysis of studies in which bees were exposed to combinations of agrochemicals, nutritional stressors and/or parasites revealed evidence for synergistic effects on mortality when bees were exposed to multiple agrochemicals at field-realistic levels.

Complex genomic alterations segregate melanoma into different molecular subsets, but for most subsets it is unclear whether they drive a distinct clinical behaviour. Here, the authors use gene-expression data from melanoma patients to search for outlier genes that correlate with survival and identify that MTSS1 is associated with metastasis.

Simultaneous PET-MRI measurements provide fundamental insights into the modulation of neural networks mediated by nicotinic acetylcholine receptors in human obesity as a putative biological mechanism for future weight loss interventions.

Milton Fernandes et al. combine single cell RNA-sequencing and functional assays to show that human oligodendrocytes exhibit an age-dependent resistance to apoptosis. Their findings have important implications for the pathology and treatment of myelination disorders like multiple sclerosis.

The temperature-sensitive miscibility of hydrocarbon, silicone and fluorocarbon liquids is used to establish a one-step method of making three- and four-phase complex emulsions with highly controllable morphologies that can be alternated between encapsulated and Janus configurations by varying the balance of interfacial tensions.

Ice flow dynamics in Greenland’s outlet glaciers are influenced by elastic deformation, both in the area of tidal influence up to 14 km inland from the grounding line and further upstream, suggest analyses of GPS observations and numerical simulations.

Dörnte et al. analyze whether T cell memory towards the B.1.1.529 spike protein of SARS-CoV-2 is retained in individuals vaccinated against the original Wuhan strain of SARS-CoV-2. CD4 and CD8 responses are slightly reduced compared to responses to the original spike protein.

This protocol describes an in vivo and ex vivo electrophysiological study in the mouse heart to derive intracardiac electrograms, focusing on electrical stimulation protocols to characterize the cardiac conduction system and its vagal control and susceptibility to arrhythmia.

The leaf microbiome compositions of 267 Arabidopsis thaliana populations across Europe reveal associations with climate and plant genetics.

Active nematics refers to systems made of a collection of elongated units, each of which consumes ambient or stored energy in order to move. The authors experimentally and numerically study an active nematic system in confinement finding a defect-free regime of shear flow, and defect nucleation under certain boundary conditions, highlighting the importance of topological defects in controlling confined active flows.

This Perspective offers guidance for robust and reproducible antibody-based highly multiplexed tissue imaging.