Cryogenic electron microscopy (cryo-EM) single-particle analysis is a powerful method for macromolecular structure determination, however, its effectiveness is often constrained by limited particle numbers or missing views. Here, the authors introduce CoCoFold, a framework integrating cryo-EM images with AlphaFold, employing a fused attention mechanism for enhanced atomic model prediction and a differentiable network for end-to-end refinement against experimental data.
- Junwen Liao
- Dihan Zheng
- Chenglong Bao
