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Showing 1–11 of 11 results
Advanced filters: Author: Keriann M. Backus Clear advanced filters

  • Mapping binding sites using photoaffinity labelling (PAL) is highly valuable but challenging for probe and drug discovery campaigns. Now a chemoproteomic approach has been developed that incorporates cleavable silyl ether linkages into PAL probes to pinpoint and quantify the precise sites where small molecules engage protein binding partners.

    • Chau Ngo
    • Sho Takechi
    • Keriann M. Backus
    ResearchOpen Access
    Nature Chemistry
    P: 1-12

  • A study established a photocatalytic proximity interactomic platform tailored to lipid droplets. This platform uses custom photosensitizers to enable in situ labeling, allowing researchers to identify contact sites of lipid droplets with other organelles in living cells without the need for genetic engineering.

    • Elijah Biletch
    • Andrew P. Becker
    • Keriann M. Backus
    News & Views
    Nature Chemical Biology
    P: 1-2

  • Becker et. al developed a proteomic proximity labeling platform named POCA, which makes use of a photosensitizer for singlet oxygen production and protein capture in the presence of amine, enabling profiling of interactomes of proteins and lipids in living cells.

    • Andrew P. Becker
    • Elijah Biletch
    • Keriann M. Backus
    Research
    Nature Chemical Biology
    P: 1-11

  • A cysteine-reactive degrader of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nonstructural protein 14 (nsp14) was identified, which mediates degradation through electrophile-induced global protein ubiquitylation, proteasome activation and the formation of stress granules and aggresomes.

    • Ashley R. Julio
    • Flowreen Shikwana
    • Keriann M. Backus
    Research
    Nature Chemical Biology
    Volume: 21, P: 693-705

  • A chemical proteomic strategy has now been reported for the global profiling of lysine reactivity and ligandability. Using this approach, >9000 lysines in the human proteome were evaluated, leading to the discovery of hyper-reactive lysines, and lysines that can be targeted by electrophilic small molecules to perturb enzyme function and protein–protein interactions.

    • Stephan M. Hacker
    • Keriann M. Backus
    • Benjamin F. Cravatt
    Research
    Nature Chemistry
    Volume: 9, P: 1181-1190

  • Cancer genomes are rife with genetic variants, and one key outcome of this variation is widespread gain-of-cysteine mutations. Here, the authors pair cysteine chemoproteomics with genomics to investigate the landscape of cysteine genetic variation.

    • Heta Desai
    • Katrina H. Andrews
    • Keriann M. Backus
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-24

  • Current methods for detection of tuberculosis rely mostly on bacterial culture from sputum. Here, the authors provide preclinical evidence that a positron-emitting mimic of the disaccharide trehalose ([18F]FDT) can be used as a radiotracer for the imaging of tuberculosis-associated lesions and monitoring the effects of treatment.

    • R. M. Naseer Khan
    • Yong-Mo Ahn
    • Benjamin G. Davis
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-19

  • Small molecules are powerful tools for investigating protein function, and can serve as leads for new therapeutics, but most human proteins lack known small-molecule ligands; here, a quantitative analysis of cysteine-reactive small-molecule fragments screened against thousands of proteins is reported.

    • Keriann M. Backus
    • Bruno E. Correia
    • Benjamin F. Cravatt
    Research
    Nature
    Volume: 534, P: 570-574

  • The use of synthetic analogs to explore substrate promiscuity during trehalose incorporation into the mycobacterial cell wall yields a fluorescent probe that can be used to examine M. tuberculosis cell biology and detect this harmful pathogen within macrophages.

    • Keriann M Backus
    • Helena I Boshoff
    • Benjamin G Davis
    Research
    Nature Chemical Biology
    Volume: 7, P: 228-235

  • Mass spectrometry-based quantitative chemoproteomics is widely used for the identification of protein targets as well as modified residues, however, sample preparation and data analysis remain tedious. Here, the authors develop silane-based cleavable linkers functionalized tandem mass tags as click-compatible isobaric tags, introducing the isobaric label earlier in sample preparation, achieving decreased sample preparation time, with high coverage and high-accuracy quantification.

    • Nikolas R. Burton
    • Keriann M. Backus
    ResearchOpen Access
    Communications Chemistry
    Volume: 7, P: 1-12

  • Communications Chemistry is pleased to introduce a Collection of research works focused on recent developments within the interdisciplinary field of Covalent chemical probes. Here, the Guest Editors highlight key themes and look towards the future of this research field.

    • Keriann M. Backus
    • Andrew J. Wilson
    EditorialOpen Access
    Communications Chemistry
    Volume: 8, P: 1-2