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Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2

Single-molecule FRET measurements provide detailed insights into the conformational states and dynamics of human Argonaute 2 that are required for its function at the core of the eukaryotic RNA silencing pathway.

Sarah Willkomm
Leonhard Jakob
Dina Grohmann
ResearchOpen Access02 Jul 2022
Nature Communications

Volume: 13, P: 1-13
DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability

TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor are important constituents of all eukaryotic initiation complexes. Here, the authors use a DNA origami-based force clamp to investigate the assembly dynamics of human initiation complexes in the RNAP II and RNAP III systems at the single-molecule level under pico newton forces.

Kevin Kramm
Tim Schröder
Dina Grohmann
ResearchOpen Access05 Jun 2020
Nature Communications

Volume: 11, P: 1-12
Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation

Transcription initiation by RNA polymerase III requires TFIIIB, a complex formed by Brf1/Brf2, TBP and Bdp1. Here, the authors describe the crystal structure of a Brf2-TBP-Bdp1 complex bound to a DNA promoter and characterize the role of Bdp1 in TFIIIB assembly and pre-initiation complex formation.

Jerome Gouge
Nicolas Guthertz
Alessandro Vannini
ResearchOpen Access25 Jul 2017
Nature Communications

Volume: 8, P: 1-11
Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

An international blind study confirms that smFRET measurements on dynamic proteins are highly reproducible across instruments, analysis procedures and timescales, further highlighting the promise of smFRET for dynamic structural biology.

Ganesh Agam
Christian Gebhardt
Thorben Cordes
ResearchOpen Access27 Mar 2023
Nature Methods

Volume: 20, P: 523-535
ERN GENTURIS guidelines on constitutional mismatch repair deficiency diagnosis, genetic counselling, surveillance, quality of life, and clinical management

Chrystelle Colas
Léa Guerrini-Rousseau
Katharina Wimmer
ReviewsOpen Access17 Oct 2024
European Journal of Human Genetics

Volume: 32, P: 1526-1541

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Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2

DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability

Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

ERN GENTURIS guidelines on constitutional mismatch repair deficiency diagnosis, genetic counselling, surveillance, quality of life, and clinical management

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