Søndergaard et al. show that electroporation and lipofectamine-based cell transfection of cancer cell lines and primary cells can be improved by adding a small vector to a large CRISPR vector. This study presents an optimized protocol for genome engineering by increasing transfection efficiency and cell viability.
- Jonas Nørskov Søndergaard
- Keyi Geng
- Claudia Kutter
