NMR spectroscopy could be a robust and reliable method for binding detection in drug discovery, but DNA/RNA targets remain challenging to analyze. Here, the authors explore the perturbation of the ligand reactivity caused by complex formation as a binding indicator to identify best binders within mixtures of significant complexity, providing a conceptually different reactivity-based alternative within NMR screening methods.
- Laura Díaz-Casado
- Andrés G. Santana
- Juan Luis Asensio
