Interferometric scattering microscopy (iSCAT) is a label-free imaging technique that enables detection of nanoscale objects. This Primer provides an overview of the principles of iSCAT detection and imaging, factors that affect its performance, its applications in the laboratory and potential avenues for advancing the technique further.
- Naomi S. Ginsberg
- Chia-Lung Hsieh
- Vahid Sandoghdar