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Showing 1–13 of 13 results
Advanced filters: Author: Martin Pacesa Clear advanced filters

  • BindCraft, an open-source, automated pipeline for de novo protein binder design with experimental success rates of 10–100%, leverages AlphaFold2 weights to generate binders with nanomolar affinity without the need for high-throughput screening.

    • Martin Pacesa
    • Lennart Nickel
    • Bruno E. Correia
    ResearchOpen Access
    Nature
    Volume: 646, P: 483-492

  • A deep learning approach enables accurate computational design of soluble and functional analogues of membrane proteins, expanding the soluble protein fold space and facilitating new approaches to drug screening and design.

    • Casper A. Goverde
    • Martin Pacesa
    • Bruno E. Correia
    ResearchOpen Access
    Nature
    Volume: 631, P: 449-458

  • CRISPR enzymes require a defined protospacer adjacent motif (PAM) which can be limiting for editing applications. Here the authors recombine the PAM-interacting domain of SpRY with the N-terminus of Sc + + to generate a chimeric enzyme with highly flexible PAM preference: SpRYc.

    • Lin Zhao
    • Sabrina R. T. Koseki
    • Pranam Chatterjee
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-8

  • A detailed understanding of the catalytic target DNA cleavage mechanism by CRISPR–Cas9 has been lacking. Now the key modulating role of Mg2+ in the conformational activation of Cas9 and the influence of active site residues on the protonation state of catalytic H840 are demonstrated, ultimately unravelling the catalytic mechanism.

    • Łukasz Nierzwicki
    • Kyle W. East
    • Giulia Palermo
    Research
    Nature Catalysis
    Volume: 5, P: 912-922

  • Cryo-electron microscopy structures of Streptococcus pyogenes Cas9 in multiple DNA-bound states provide insights on the mechanism of Cas9 activation by target DNA.

    • Martin Pacesa
    • Luuk Loeff
    • Martin Jinek
    ResearchOpen Access
    Nature
    Volume: 609, P: 191-196

  • A computational deep learning approach is used to design synthetic proteins that target the neosurfaces formed by protein–ligand interactions, with applications in the development of new therapeutic modalities such as molecular glues or cell-based therapies.

    • Anthony Marchand
    • Stephen Buckley
    • Bruno E. Correia
    ResearchOpen Access
    Nature
    Volume: 639, P: 522-531

  • A surface-centric approach captures the physical and chemical determinants of molecular recognition, enabling the de novo design of protein interactions and of artificial proteins with function.

    • Pablo Gainza
    • Sarah Wehrle
    • Bruno E. Correia
    ResearchOpen Access
    Nature
    Volume: 617, P: 176-184

  • Through directed evolution, the PAM compatibility of the compact Cas9 variant CjCas9 was increased. Evolved CjCas9 shows higher nuclease activity at canonical and non-canonical sites and enables robust in vivo gene editing from single AAV vectors.

    • Lukas Schmidheini
    • Nicolas Mathis
    • Gerald Schwank
    Research
    Nature Chemical Biology
    Volume: 20, P: 333-343