An enzyme electrode was fabricated by covalent immobilization of acid phosphatase (ACP) on a polyaniline/poly(acrylic acid) composite film for use as the anode of a fuel cell driven with L-ascorbic acid 2-phosphate (ASA2P). The enzyme electrode showed an activity in biochemical dephosphorylation of ASA2P to ascorbic acid (ASA), and succeeded in electrochemical oxidation of ASA. The ASA2P fuel cell equipped with the enzyme electrode gave a maximum power output of 6.0 μW cm−2-anode, reflecting the bioelectrochemical activity of the electrode.
- Toshimasa Homma
- Mizuki Kondo
- Masato Shimomura