Determining the appropriate sample size (N) for bulk RNA sequencing experiments is critical to ensure reliable results. Here the authors perform an unusually large N experiment (N = 30 per group), analyzing changes in gene expression in two genetically modified mice compared to controls. They find that a surprisingly high N is required to keep the false positive rate below 50% and detection sensitivity above 50%.
- Gabor Halasz
- Jennifer Schmahl
- David J. Glass
