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Showing 1–6 of 6 results
Advanced filters: Author: Orane Guillaume-Gentil Clear advanced filters

  • Live-seq, a single-cell transcriptome profiling approach that preserves cell viability during RNA extraction using fluidic force microscopy, can address a range of biological questions by transforming scRNA-seq from an end-point to a temporal analysis approach.

    • Wanze Chen
    • Orane Guillaume-Gentil
    • Bart Deplancke
    ResearchOpen Access
    Nature
    Volume: 608, P: 733-740

  • The adoption of microfluidics was fundamental to the development of cost-effective, high-throughput DNA sequencing. As the field progresses towards multi-omics, Lambert et al. reflect on the key concepts underlying microfluidics and how resulting engineering advances at the microscale drove the evolution of genomic sequencing.

    • Camille L. G. Lambert
    • Guido van Mierlo
    • Bart Deplancke
    Comments & Opinion
    Nature Reviews Genetics
    Volume: 26, P: 1-2

  • Fluidic force microscopy combines atomic force microscopy with microfluidic probes to enable measurement and manipulation of materials at sub-micrometre resolution. In this Primer, Zambelli et al. discuss the principles of fluidic force microscopy and applications in biological research and nanotechnology.

    • Tomaso Zambelli
    • Orane Guillaume-Gentil
    • Julia A. Vorholt
    Reviews
    Nature Reviews Methods Primers
    Volume: 6, P: 1-21

  • Guillaume-Gentil et al. describe a method that employs a modified AFM tip for selectively sampling from and injecting into individual fungal cells of differing morphology. The authors describe extensive modifications on their system previously used for mammalian cells to overcome many of the challenges associated with working on single fungal cells.

    • Orane Guillaume-Gentil
    • Christoph G. Gäbelein
    • Julia A. Vorholt
    ResearchOpen Access
    Communications Biology
    Volume: 5, P: 1-10

  • A study presents an approach to establish and track a new endosymbiotic partnership by implanting bacteria in a non-host fungus and shows that stable inheritance of the implanted bacteria is possible with positive selection.

    • Gabriel H. Giger
    • Chantal Ernst
    • Julia A. Vorholt
    ResearchOpen Access
    Nature
    Volume: 635, P: 415-422

  • Research is moving from a gene- and protein-centric view toward a holistic understanding of cellular phenotypes. This Review discusses the technological microfluidics challenges that must be addressed to realize integrative single-cell phenomics.

    • Camille L. G. Lambert
    • Guido van Mierlo
    • Bart Deplancke
    Reviews
    Nature Genetics
    Volume: 57, P: 1344-1356