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Fluorescent probes for bioimaging need to exhibit bright fluorescence, be biocompatible and offer several alternatives for attachment to biomolecules of interest. Here, a near-infrared silicon–rhodamine fluorophore is introduced that can be coupled to intracellular proteins in live cells and tissues and can be exploited for super-resolution microscopy.

Structural, biochemical and mutagenesis studies indicate that, in dying cells, the membrane protein NINJ1 assembles into filaments, disrupting the cell membrane.

Mitochondrial Ndufs4, a subunit of complex I, is a regulator of the electron transport chain. Here, the authors show that forced expression of Ndufs4 in podocytes improves the assembly of respiratory supercomplexes, maintains cristae integrity, and mitigates the progression of diabetic kidney disease

Coherent patterns in the initial growth of extratropical peatlands throughout the Southern Hemisphere during the last glacial track changes in the latitudinal position of the southern westerly winds, according to an analysis of basal peat radiocarbon ages.

In budding yeast, glucose withdrawal, via the Rag GTPases, leads to TORC1 inhibition through its re-organization into a giant, vacuole-associated helix named a TOROID (TORC1 organized in inhibited domain).

Rathmell and colleagues show that metabolic reprograming of regulatory T cells is associated with severity of critical illness in patients with and without sepsis.

An assessment of blue carbon strategies in Belize shows how quantifying fisheries, tourism and coastal risk co-benefits alongside carbon benefits can inform spatial and temporal target setting for nationally determined climate contributions that simultaneously provide societal benefits.

Targeting histone deacetylases (HDACs) alone has shown limited success in solid tumours. Here, authors report that the HDAC1/2 inhibitor romidepsin confers responsiveness to receptor tyrosine kinase inhibitors, with enhanced therapeutic effects in models of hepatocellular carcinoma, leading to tumour regression and an immune-stimulatory profile.

Future changes and regional differences in snowpacks are unclear. Here the American Cordillera mountain range, spanning the Americas, is estimated to lose snow faster in the southern midlatitudes—global warming should be limited to below 2.5 °C to prevent low-to-no-snow conditions across the range.

Economic estimates of flood damages rely on depth–damage functions that are inadequately verified. Here, the authors assessed flood vulnerability in the US and found that current depth–damage functions consist of disparate relationships that match poorly with observations which better follow a bimodal beta distribution.

The magnetic flux lines in a superconductor present intricate patterns, whose origins are seldom understood. Here the authors link them to geometrical effects by means of the 'hairy ball' theorem, which states that for a vector field on a sphere there will always be at least one singularity.

Many RNA viruses that replicate in the cytoplasm express proteins that localize to nucleoli, but the nucleolar functions remain largely unknown. Here, the authors show that the Henipavirus matrix protein mimics an endogenous Treacle partner of the DNA-damage response, resulting in suppression of rRNA biogenesis.

While therapies targeting type I BRAF mutations have been developed, there are limited options for those with type II and III mutations. Here, the authors identify a subset of BRAF-mutant non-small cell lung cancer patients and characterise the pan-RAF inhibitor exarafenib, demonstrating efficacy in preclinical models and investigating subsequent resistance mechanisms.

The evolution of oceanic redox state in the past is poorly known. Here, the authors present a temporal record of banded iron formations and marine red beds, which indicate deep-ocean oxygenation occurred in the middle Ediacaran, coinciding with the onset of widespread marine red beds.

Multifocal flat illumination for field-independent imaging (mfFIFI) enables patterned illumination over an extended field of view. Integration with instant structured illumination microscope allowed for high-speed, multicolor, volumetric super-resolution imaging over 100 × 100 µm².

The variability in clinical outcomes of SARS-CoV-2 infection is partly due to deficiencies in production or response to type I interferons (IFN). Here, the authors describe a FIP200-dependent lysosomal degradation pathway, independent of canonical autophagy and type I IFN, that restricts SARS-CoV-2 replication, offering insights into critical COVID-19 pneumonia mechanisms.

Alpha-synuclein is associated with neuronal dysfunction in Parkinson’s disease. This study shows that alpha-synuclein interacts with neuronal synaptic vesicles in a calcium-dependent fashion, and this interaction is important for synaptic vesicle clustering.

Integrins can regulate antigen-specific and innate immune receptor signalling, thereby affecting immune cell function. Here the authors show that avß3 integrin controls Toll-like receptor (TLR) signalling by regulating its trafficking to limit TLR-mediated B-cell proliferation and antibody production.

Mars Climate Sounder’s multi-annual observations of the vertical distribution of water and dust in the Martian atmosphere show that deep convection from dust storms transports water from the lower to the middle atmosphere, enhancing water loss to space.

cellSTAAR advances noncoding rare variant association analysis by integrating single-cell genomics data.

Elevated viscosity counterintuitively increases the motility of various cell types in vitro and imprints mechanical memory to tumour cells, which enables them to disseminate more efficiently in vivo.

Convalescent plasma therapy (CPT) is used to treat patients with Covid-19, but evidence for clinical benefit from clinical trials is divergent. Here the authors report in that CPT treatment does not affect mortality in patients with severe Covid-19 in phase 2 single center open label randomised controlled trial.

The non-linear interaction between tide and non-tidal residual impacts current and future extreme water levels. Here, based on 620 gauge records, the authors find a large non-linear interaction in the US East Coast, North Sea and parts of southern Japan, that results in a reduction of extreme sea levels.

Petrels are wide-ranging, highly threatened seabirds that often ingest plastic. This study used tracking data for 7,137 petrels of 77 species to map global exposure risk and compare regions, species, and populations. The results show higher exposure risk for threatened species and stress the need for international cooperation to tackle marine litter.

This study shows that conserving approximately half of global land area through protection or sustainable management could provide 90% of ten of nature’s contributions to people and could meet representation targets for 26,709 species of mammals, birds, amphibians, and reptiles. This finding supports recent commitments to conserve at least 30% of global lands and waters by 2030.

Whole-genome sequencing analysis of individuals with primary immunodeficiency identifies new candidate disease-associated genes and shows how the interplay between genetic variants can explain the variable penetrance and complexity of the disease.

El Niño events systematically reduce photovoltaic power potential across major solar regions, with Super El Niño episodes triggering multimonth solar energy deficits and temporary increases in carbon dioxide emissions, according to four decades of reanalysis data.

A 90-year sea level record (1930-2019) is constructed from the growth of a tropical coral and reveals a marked acceleration in sea-level rise in the central Indian Ocean beginning in 1959 (3.44 ± 0.68 mm.y-1), much earlier than previously thought.

A transition from microbial-dominated Ediacara biota into modern ecosystems marks the beginning of the Cambrian. Here, Buatois et al.describe Ediacaran microbial mats in an early Cambrian formation in Canada suggesting that Ediacara biota persisted in the early Cambrian and abruptly disappeared later on.

2024 was one of the wettest years on record, witnessing record-breaking extreme precipitation events across the globe, several of which were compound events. Extreme rainfalls were unprecedented in arid regions and parts of the Global South, with severe monsoon rains and rapidly intensifying tropical cyclones causing severe casualties and economic losses.

Ageing of the immune system is now realized to drive systemic ageing, and there is interest in targeting immune ageing in order to promote healthy ageing. Here, the authors detail how ageing affects different immune cell populations and discuss strategies to rejuvenate the immune system in order to extend healthspan.

Podosomes are actin-based protrusions used by cells for invasion and local degradation but the structure underlying their protrusiveness and mechanosensitivity is unclear. Here, the authors report that podosomes have a modular actin nano-architecture whose organization differs on stiff or soft substrates.

The authors collate literature on the responses of bird assemblages to forest loss and show that locations with a more variable natural environment and a longer history of agricultural land use have bird assemblages that are more tolerant to forest loss.

For over 70 years, almost every drug approved for schizophrenia has modulated dopamine signalling, thus only addressing some symptoms and having notable side effects. Here, Coyle and Paul discuss drugs with promising new mechanisms under development for schizophrenia, including those targeting glutamatergic and cholinergic signalling.

Blockade of the C5a–C5aR1 axis using anti-C5aR1 monoclonal antibodies prevented inflammation associated with COVID-19.

Inundation and erosion could make many atoll islands uninhabitable over the next century. Here the authors present an analysis of change in the atoll nation of Tuvalu that shows a 2.9% increase in land area over the past four decades, with 74% of islands increasing in size, despite rising sea levels.

Current expansion microscopy approaches need further optimization to achieve the precision of nanoscopy techniques. Here, the authors develop an iterative ultrastructure expansion microscopy (iU-ExM) approach that achieves SMLM-level resolution.

A generalizable, functional-trait-based approach for quantifying the effects of disturbances to ecosystem services and economic outcomes, including under climate change, highlights the need for incorporating disturbances in ecosystem services assessments.

Current short-pulse-duration neutron sources suffer from a low repetition rate, hindering applications. Here, the authors demonstrate advancements of laser-wakefield based photoneutron generation at high repetition rates and conversion efficiencies, providing an alternative to traditional pitcher-catcher methods.